Review



recombinant human keap1 protein  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 93
      Buy from Supplier

    Structured Review

    Sino Biological recombinant human keap1 protein
    Recombinant Human Keap1 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/Human+KEAP1+INRF2+Protein/10__1016_slash_j__phytol__2025__103035-103-25-32
    Average 93 stars, based on 25 article reviews
    recombinant human keap1 protein - by Bioz Stars, 2026-10
    93/100 stars

    Images

    Related Articles

    Recombinant:

    Article Title: Regulation of NRF2 by Stably associated Phosphoinositides and Small Heat Shock Proteins in Response to Stress.
    Article Snippet: .. Recombinant KEAP1 protein (#11981-H20B) was purchased from Sino Biological. .. The binding assay was performed in PBST by incubating a constant amount of His-tagged NRF2 with an increasing amount of HSP27 or αBcrystallin in the presence of 20 μl anti-NRF2 antibody-conjugated agarose (#sc-365949 AC, Santa Cruz) at 4°C overnight.

    Article Title: Regulation of NRF2 by stably associated phosphoinositides and small heat shock proteins in response to stress
    Article Snippet: .. Recombinant KEAP1 protein (#11981-H20B) was purchased from Sino Biological. .. The binding assay was performed in PBST by incubating a constant amount of His-tagged NRF2 with an increasing amount of HSP27 or αB-crystallin in the presence of 20 μl anti-NRF2 antibody–conjugated agarose (#sc-365949 AC; Santa Cruz) at 4 °C overnight.

    Article Title: Regulation of NRF2 by Phosphoinositides and Small Heat Shock Proteins
    Article Snippet: .. Recombinant KEAP1 protein (#11981-H20B) was purchased from Sino Biological. .. The binding assay was performed in PBST by incubating a constant amount of His-tagged NRF2 with an increasing amount of HSP27 or αB-crystallin in the presence of 20 μl anti-NRF2 antibody-conjugated agarose (#sc-365949 AC, Santa Cruz) at 4°C overnight.

    Article Title: Withaferin A induces heme oxygenase (HO-1) expression in endothelial cells via activation of the Keap1/Nrf2 pathway.
    Article Snippet: Neutravidin agarose beads were purchased from Thermo Scientific (Rockford, IL), Protein A on Trisacryl beads from Pierce Protein Biology (Rockford, IL). .. Recombinant Keap1-protein was obtained from Sino Biological (Beijing, China). .. Trypsin was purchased from Promega (Madison, USA).

    Article Title: Rosmarinic acid prevents refractory bacterial pneumonia through regulating Keap1/Nrf2-mediated autophagic pathway and mitochondrial oxidative stress.
    Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA) is the leading cause of bacterial pneumonia, featured with exuberant inflammatory cytokine production, extensive oxidative stress and tissue injury.. The Keap1/Nrf2 system is the major apparatus essential for host defense against oxidative and electrophilic stresses of both exogenous and endogenous origins, representing a logical target for host-directed strategy to treat severe inflammatory diseases including MRSA-induced pneumonia.. In an effort to search therapeutics for bacterial pneumonia, we identify rosmarinic acid (RA) as a covalent modifier of Keap1 and hence an activator of Nrf2.



    Similar Products

    s8790 keap1 targetmol cat  (TargetMol)
    93
    TargetMol s8790 keap1 targetmol cat
    S8790 Keap1 Targetmol Cat, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/KEAP1+Protein%2C+Human%2C+Recombinant/pm41146891-52-142-144
    Average 93 stars, based on 1 article reviews
    s8790 keap1 targetmol cat - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    human recombinant keap1 protein  (MedChemExpress)
    94
    MedChemExpress human recombinant keap1 protein
    Human Recombinant Keap1 Protein, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/KEAP1%2C+Human/10__1016_slash_j__phymed__2025__157293-160-0-4
    Average 94 stars, based on 1 article reviews
    human recombinant keap1 protein - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    recombinant human keap1 protein  (Sino Biological)
    93
    Sino Biological recombinant human keap1 protein
    Recombinant Human Keap1 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/Human+KEAP1+INRF2+Protein/10__1016_slash_j__phytol__2025__103035-103-25-32
    Average 93 stars, based on 1 article reviews
    recombinant human keap1 protein - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    tmpy  (TargetMol)
    93
    TargetMol tmpy
    Tmpy, supplied by TargetMol, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/KEAP1+Protein%2C+Human%2C+Recombinant/pm41146891-52-147-144
    Average 93 stars, based on 1 article reviews
    tmpy - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    recombinant keap1 protein keap1 ddk  (OriGene)
    93
    OriGene recombinant keap1 protein keap1 ddk
    Recombinant Keap1 Protein Keap1 Ddk, supplied by OriGene, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/Keap1+(NM_001110307)+Mouse+Recombinant+Protein/pm23773140__cb4000103_si_001-145-0-6
    Average 93 stars, based on 1 article reviews
    recombinant keap1 protein keap1 ddk - by Bioz Stars, 2026-10
    93/100 stars
    		  Buy from Supplier
    recombinant keap1 protein  (Sino Biological)
    94
    Sino Biological recombinant keap1 protein
    HSP27 stabilizes NRF2. A , MDA-MB-231 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. B , RT–PCR analysis of NFE2L2 mRNA in MDA-MB-231 cells. NFE2L2 mRNA levels were normalized to GAPDH mRNA. The graph is shown as mean ± SD of n = 4 independent experiments. C , MDA-MB-231 cells transfected with control siRNAs or siRNAs targeting HSP27 were treated with or without 10 μM MG132 for the indicated time. NRF2 protein levels were determined by IB. D and E , HCT116 ( D ) or HS578 ( E ) cells were transiently transfected with control siRNAs or siRNAs targeting HSP27. About 68 h later, transfected cells were treated with vehicle or 100 μM tBHQ for an additional 4 h, and NRF2 expression was then analyzed by IB. The graph is shown as mean ± SD. F , A549 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. G , 0.5 μg of recombinant His-tagged NRF2 and 9 μg <t>KEAP1</t> were incubated with the indicated concentrations of HSP27. NRF2 was pulled down, and the bound KEAP1 and HSP27 were analyzed by IB. For all panels , n = 3 independent experiments unless otherwise indicated. HSP27, heat shock protein 27; IB, immunoblotting; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2–related factor 2; tBHQ, tertiary butylhydroquinone.
    Recombinant Keap1 Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/Human+KEAP1+%2F+INRF2+Protein/pmc12273567-228-0-7
    Average 94 stars, based on 1 article reviews
    recombinant keap1 protein - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    his gst keap1 recombinant protein  (Sino Biological)
    94
    Sino Biological his gst keap1 recombinant protein
    HSP27 stabilizes NRF2. A , MDA-MB-231 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. B , RT–PCR analysis of NFE2L2 mRNA in MDA-MB-231 cells. NFE2L2 mRNA levels were normalized to GAPDH mRNA. The graph is shown as mean ± SD of n = 4 independent experiments. C , MDA-MB-231 cells transfected with control siRNAs or siRNAs targeting HSP27 were treated with or without 10 μM MG132 for the indicated time. NRF2 protein levels were determined by IB. D and E , HCT116 ( D ) or HS578 ( E ) cells were transiently transfected with control siRNAs or siRNAs targeting HSP27. About 68 h later, transfected cells were treated with vehicle or 100 μM tBHQ for an additional 4 h, and NRF2 expression was then analyzed by IB. The graph is shown as mean ± SD. F , A549 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. G , 0.5 μg of recombinant His-tagged NRF2 and 9 μg <t>KEAP1</t> were incubated with the indicated concentrations of HSP27. NRF2 was pulled down, and the bound KEAP1 and HSP27 were analyzed by IB. For all panels , n = 3 independent experiments unless otherwise indicated. HSP27, heat shock protein 27; IB, immunoblotting; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2–related factor 2; tBHQ, tertiary butylhydroquinone.
    His Gst Keap1 Recombinant Protein, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/Human+KEAP1+%2F+INRF2+Protein/pmc10374160__pnas__2217128120__sapp-24-49-52
    Average 94 stars, based on 1 article reviews
    his gst keap1 recombinant protein - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    recombinant keap1 protein  (TargetMol)
    94
    TargetMol recombinant keap1 protein
    HSP27 stabilizes NRF2. A , MDA-MB-231 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. B , RT–PCR analysis of NFE2L2 mRNA in MDA-MB-231 cells. NFE2L2 mRNA levels were normalized to GAPDH mRNA. The graph is shown as mean ± SD of n = 4 independent experiments. C , MDA-MB-231 cells transfected with control siRNAs or siRNAs targeting HSP27 were treated with or without 10 μM MG132 for the indicated time. NRF2 protein levels were determined by IB. D and E , HCT116 ( D ) or HS578 ( E ) cells were transiently transfected with control siRNAs or siRNAs targeting HSP27. About 68 h later, transfected cells were treated with vehicle or 100 μM tBHQ for an additional 4 h, and NRF2 expression was then analyzed by IB. The graph is shown as mean ± SD. F , A549 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. G , 0.5 μg of recombinant His-tagged NRF2 and 9 μg <t>KEAP1</t> were incubated with the indicated concentrations of HSP27. NRF2 was pulled down, and the bound KEAP1 and HSP27 were analyzed by IB. For all panels , n = 3 independent experiments unless otherwise indicated. HSP27, heat shock protein 27; IB, immunoblotting; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2–related factor 2; tBHQ, tertiary butylhydroquinone.
    Recombinant Keap1 Protein, supplied by TargetMol, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/TNF-alpha%2C+recombinant+human+protein/pm39862944-56-2-22
    Average 94 stars, based on 1 article reviews
    recombinant keap1 protein - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    keap1 protein, human, recombinant  (TargetMol)
    90
    TargetMol keap1 protein, human, recombinant
    HSP27 stabilizes NRF2. A , MDA-MB-231 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. B , RT–PCR analysis of NFE2L2 mRNA in MDA-MB-231 cells. NFE2L2 mRNA levels were normalized to GAPDH mRNA. The graph is shown as mean ± SD of n = 4 independent experiments. C , MDA-MB-231 cells transfected with control siRNAs or siRNAs targeting HSP27 were treated with or without 10 μM MG132 for the indicated time. NRF2 protein levels were determined by IB. D and E , HCT116 ( D ) or HS578 ( E ) cells were transiently transfected with control siRNAs or siRNAs targeting HSP27. About 68 h later, transfected cells were treated with vehicle or 100 μM tBHQ for an additional 4 h, and NRF2 expression was then analyzed by IB. The graph is shown as mean ± SD. F , A549 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. G , 0.5 μg of recombinant His-tagged NRF2 and 9 μg <t>KEAP1</t> were incubated with the indicated concentrations of HSP27. NRF2 was pulled down, and the bound KEAP1 and HSP27 were analyzed by IB. For all panels , n = 3 independent experiments unless otherwise indicated. HSP27, heat shock protein 27; IB, immunoblotting; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2–related factor 2; tBHQ, tertiary butylhydroquinone.
    Keap1 Protein, Human, Recombinant, supplied by TargetMol, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+keap1+protein/KEAP1+Protein%2C+Human%2C+Recombinant/targetmol___tmpy-03888
    Average 90 stars, based on 1 article reviews
    keap1 protein, human, recombinant - by Bioz Stars, 2026-10
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    HSP27 stabilizes NRF2. A , MDA-MB-231 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. B , RT–PCR analysis of NFE2L2 mRNA in MDA-MB-231 cells. NFE2L2 mRNA levels were normalized to GAPDH mRNA. The graph is shown as mean ± SD of n = 4 independent experiments. C , MDA-MB-231 cells transfected with control siRNAs or siRNAs targeting HSP27 were treated with or without 10 μM MG132 for the indicated time. NRF2 protein levels were determined by IB. D and E , HCT116 ( D ) or HS578 ( E ) cells were transiently transfected with control siRNAs or siRNAs targeting HSP27. About 68 h later, transfected cells were treated with vehicle or 100 μM tBHQ for an additional 4 h, and NRF2 expression was then analyzed by IB. The graph is shown as mean ± SD. F , A549 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. G , 0.5 μg of recombinant His-tagged NRF2 and 9 μg KEAP1 were incubated with the indicated concentrations of HSP27. NRF2 was pulled down, and the bound KEAP1 and HSP27 were analyzed by IB. For all panels , n = 3 independent experiments unless otherwise indicated. HSP27, heat shock protein 27; IB, immunoblotting; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2–related factor 2; tBHQ, tertiary butylhydroquinone.

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of NRF2 by stably associated phosphoinositides and small heat shock proteins in response to stress

    doi: 10.1016/j.jbc.2025.110367

    Figure Lengend Snippet: HSP27 stabilizes NRF2. A , MDA-MB-231 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. B , RT–PCR analysis of NFE2L2 mRNA in MDA-MB-231 cells. NFE2L2 mRNA levels were normalized to GAPDH mRNA. The graph is shown as mean ± SD of n = 4 independent experiments. C , MDA-MB-231 cells transfected with control siRNAs or siRNAs targeting HSP27 were treated with or without 10 μM MG132 for the indicated time. NRF2 protein levels were determined by IB. D and E , HCT116 ( D ) or HS578 ( E ) cells were transiently transfected with control siRNAs or siRNAs targeting HSP27. About 68 h later, transfected cells were treated with vehicle or 100 μM tBHQ for an additional 4 h, and NRF2 expression was then analyzed by IB. The graph is shown as mean ± SD. F , A549 cells were transiently transfected with control siRNAs or siRNAs targeting HSP27, and NRF2 expression was analyzed by IB 72 h later. The graph is shown as mean ± SD. G , 0.5 μg of recombinant His-tagged NRF2 and 9 μg KEAP1 were incubated with the indicated concentrations of HSP27. NRF2 was pulled down, and the bound KEAP1 and HSP27 were analyzed by IB. For all panels , n = 3 independent experiments unless otherwise indicated. HSP27, heat shock protein 27; IB, immunoblotting; KEAP1, Kelch-like ECH-associated protein 1; NRF2, nuclear factor erythroid 2–related factor 2; tBHQ, tertiary butylhydroquinone.

    Article Snippet: Recombinant KEAP1 protein (#11981-H20B) was purchased from Sino Biological.

    Techniques: Transfection, Control, Expressing, Reverse Transcription Polymerase Chain Reaction, Recombinant, Incubation, Western Blot